waters sep-pak c18 cartridge protocol

Found inside – Page 107Preparation of tissue samples Broadly, this protocol is similar to the one described in ... Load the supernatants onto Sep-Pak C18 cartridges (Waters). Found inside – Page 650Human volunteer study After approval of the protocol by the Institutional Review Board , informed consent was obtained from three ... the urine samples were applied to Sep - Pak C18 cartridges ( Waters Chromatography Division , Millipore Corporation , Milford , MA , USA ) ... The supernatant was extracted with Sep - Pak C18 cartridge and processed as for the urine samples and analysed by h . p . l . c . Found insideInorganic 36C1 was removed using Sep-Pak C18 cartridges (Waters Associates, Milford, MA). The cartridges were conditioned with 10 mL of methanol, ... Found inside – Page 397The water phase is dried, dissolved in 10% trichloroacetic acid, and filtered through C18 Sep-Pak cartridges (Waters), which are preconditioned with 5 ml ... Found inside – Page 440A simple and fast protocol for ABA analysis developed by our group is as follows: ... pH 7.8) and later passed through a Waters Sep-Pak C18 cartridge. Found inside – Page 68... passed through a Waters Sep-Pak cartridge that had been previously washed with 5 ... Samples are chromatographed on a Waters Radial-Pak C18 column (on a ... Found inside – Page 374The Sep - Pak cartridge described here has a peptide capacity of 5 mg . The procedure ( s ) described in this chapter can be scaled down in reasonable fashion . For example , Waters sells Sep - Pak cartridges labeled “ 1 cc , ” which have a ... Found inside – Page 165... 2013 were further purified using C18 cartridges (Sep-Pak, Waters, Dublin, ... according to the recommended protocol (ABRAXIS, Warminster, PA, USA). Found inside – Page 211Protocol of the σ1 Receptor Binding Assay According to [35–37]: the assay was performed ... Sep-Pak® C18 cartridges Plus, Plus short and Plus light (Waters, ... Comprehensive and versatile, Pesticide Protocols offers analysts powerful and highly practical tools for analyzing the presence of pesticides in complex matrices. Found inside – Page 71The fraction was purified using ODS extraction mini column (C18 Sep-Pak Cartridge, Waters) with water followed by evaporation to dryness. Found inside – Page 143Protocol 3. Desalting oligonucleoside methylphosphonates 1. Attach a SEP PAK C18 reversed phase cartridge ( Waters Inc. ) to a threeway nylon valve ( BioRad Inc ) which is fitted to the end of a 10 ml syringe . 2. Pre - equilibrate the SEP ... Found inside – Page 11Fractionation of polyphenolics into non - anthocyanin and anthocyanin fractions using C18 Sep - Pak cartridges ... ( see Basic Protocol 1 or Alternate Protocol 1 ) Nitrogen gas C18 Sep - Pak cartridges ( Waters Chromatography ) 1 1 0.45 - um ... Found inside – Page 435Sep - Pak C18 reversed - phase cartridges were from Waters Associates ( Milford ... of MCYST content in BGA samples according to the protocol of Chu et al . Found inside – Page 231This protocol outlines the steps for buffer exchange of purified DNA using a Sep-Pak C18 cartridge from Waters Corporation. 1. Attach C18 column to a 10-mL ... Found inside – Page 96Use a Waters Sep-Pak® C18 or tC18 cartridge (Millipore) according to the volume of sample solution and then follow Protocol 4, substituting the word ... Found inside – Page 61 I PROTOCOL SUMMARY 1 Polarity - Class Fractionation 1 1 Miniature reverse - phase cartridges are available from several manufacturers ( e.g. , C18 SEP PAKs from Waters Assoc . , Inc. , Milford , MA ; Disposable Extraction Columns from ... Found inside – Page 148Separate the labelled peptide from free radiolabel by reverse phase chromatography on a disposable C18 cartridge ( e.g. Sep - Pak , from Waters ) . Found inside – Page 300... through a C18 cartridge or minicolumn ( e . g . , Fisher Prep - Sep Cis ; Waters Sep - Pak Cıs ) . One protocol is described in Section II . Found inside – Page 178Politics , ( continued ) The Europeanisation of local environmental politics : Bathing water pollution in south - west England ..9913 The ... Protocol for the analysis of high concentrations of benzene , toluene , ethylbenzene , and xylene isomers in water using automated ... in natural water by spectrophotometry after pre - concentration using Sep - Pak C18 cartridge .9280 Determination of volatile organic ... Found inside – Page 330... that can be used on the bench (Waters Sep-Pak C18 Classic Cartridge). ... A classical protocol consists of the following steps: 1) Plug the column inlet ... Found inside – Page 96... C . The supernatant was applied to a C18 cartridge ( Sep - Pak Plus C18 Environmental , Waters , St. Quentin en Yvelines ... was purified by C18 reverse phase - high - performance liquid chromatography ( RP - HPLC ) following the protocol ... Found inside – Page 512After the precipitates were removed, the supernatant acetone was evaporated and loaded onto a 40-g cartridge of Sep-Pak C18 (Waters), ... Found inside – Page 377During these protocols the increases in content of water and Nacl of the body were ... C18 SEP - PAK® cartridges ( Waters , Millipore Corporation , USA ) . Found inside – Page 220At the last stage, the analytes are eluted from the cartridge with a solvent, ... from plasma samples by SPE using Sep-Pak C18 cartridges (500 mg, ... Found inside – Page 16... trifluoroacetic acid and desalted on C18 solid-phase extraction cartridges (SEP-PAK plus, Waters), dried, and re-suspended in Oxford, UK). Found inside – Page 1142Informed consent was obtained from each subject studied , and the study protocol conformed to the ethical guidelines of ... minutes at 4 ° C . Acidified supernatants were applied to Sep - Pak C18 cartridges ( Waters Associates , Milford , MA ) . Found inside – Page 329The sterolfractions eluted from the initial Certified Sep-Pak C18 (200 mg, Waters Corporation) column (SPE1; see above “Extraction Protocols”) are ... Found inside – Page 7133HPLC Apparatus , Solvent Systems and Elution Protocols MA ) . ... DNA adducts ) ; protocol The material extracted with butanol and C18 - Sep - Pak clean - up was B , 50 % methanol in water for 30 min ... 9 cm ) followed The BaP - modified DNA was dissolved in 10 mM Tris - HCl buffer by C18 - Sep - Pak cartridges using a ... Found inside – Page 132... HDPV with 0.22 μm pore and 13 mm filter holders • Solid phase extraction cartridges, Sep-Pack Vac, Waters (200 mg) C18 support (3 mL capacity • 20 mL ... Found inside... or by rapid HPLC using Sep-Pak® C18 cartridges (Waters).7172 Contaminants such ... and this technique is an important part of the protocol developed by ... Found inside – Page 241Then, 1 mL of diluted mixture was passed through pre-activated Sep-Pak C18 cartridges (96-well type OASIS HLB extraction cartridge, Waters). This 212 page book covers many topics including SPE device formats, sorbent considerations, mobile phase selection, and troubleshooting. Found inside – Page 84With the use of this protocol , the average recovery of the internal standard was 90.1 + ... The urine was passed through a Sep - Pak C18 cartridge ( Waters ... Found inside – Page 976... for example, by adsorption on Sep-Pak C18 cartridges (Waters Associates, Milford, MA) or by gel-permeation chromatography on a Sephadex G-10 desalting ... Found inside – Page 69of distilled water . Desalt the sample by passage through a C18 Sep - Pak cartridge ( Waters ) , i.e. , apply the sample to an activated ( 2 ml of methanol ) ... Found inside – Page 144Analysis was performed on a UPLC/ESI-MS/MS utilizing a Waters ACQUITY UPLC ... were passed through equilibrated Sep-Pak C18 cartridges (Waters, Mississauga, ... Found inside – Page 159... is purified with a series of two Sep-Pak Plus C18 cartridges (Waters, Milford, MA). ... by following the protocol described above, hapten 15 (4 mg, ... Found inside – Page 1467... + Hexane Solvent extraction Sep Pak silica cartridge cleanup Elution by n ... of PAHs in water ( 95–99 ) and airborne particulate matter ( 100-104 ) . Found inside – Page 84With the use of this protocol , the average recovery of the internal standard was 90.1 + 2.0 % ( mean + SEM ) , and all NPRO values were ... The urine was passed through a Sep - Pak C18 cartridge ( Waters Associates , Inc. , Milford , Mass . ) ... Found inside – Page 2-38UNIT 3.3 In Vitro Radiolabeling of Peptides and Proteins BASIC PROTOCOL 1 IODINATION AT TYROSINE OR ... use ) Solid - phase extraction device ( e.g. , Sep - Pak or Sep - Pak Plus C18 cartridge , Waters ) Luer - tipped syringes ( 1- , 5- ... Found inside – Page 277Sep-Pak C18 cartridge (1 cc, Waters) 9. ... support such as Affi-Gel 102 allows simple coupling using EDC according to the following protocol. Found inside – Page 197... and the mixtures incubated for 30 min at 25 ° C . A different protocol was used to study enzyme inactivation by ABT . ... after 30 min with 20 ul of 4N HCI , then diluted with 5 ml water and loaded on a C18 cartridge ( Sep - pak , Waters ) . Found inside – Page 204A very efficient way of achieving this is to use reverse-phase SepPak columns that can be used on the bench (Waters Sep-Pak C18 Classic short-body). Found inside – Page 13The superfusate samples for HPLC were processed by SEP - PAK C18 cartridge ( Waters Associates ) . The remainder of all superfusate samples from each tissue type used in a single experimental protocol were pooled and passed through a ... Found inside – Page 156The Millipore filtration step used by these workers was replaced by processing through a C18 Sep-Pak cartridge (Waters Associates, Milford, USA). Found inside – Page 813The urine was passed through a SepPak C18 Cartridge ( Waters Associates , Milford , MA , USA ) , and the cartridge was ... Using this protocol , the average recovery of the internal standard was 90.1 + 2.0 % ( mean + SE ) , and all NPRO ... Found inside – Page 1397The study was performed under a protocol approved by the animal care committees of St. Joseph's Health Centre and the ... x g for 30 min , and the supernatant was extracted on a C18 Sep - pak cartridge ( Waters Associates , Milford , Mass . ) ... 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